The King Lab (some assembly required)

Dept. of Biochemistry
Institute for Protein Design
University of Washington

King Lab | Lab Members

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PI

neilking@uw.edu

Neil King, PhD

Neil studied Biomedical Engineering as an undergraduate at Northwestern University, followed by graduate studies in Biochemistry in the lab of Todd Yeates at UCLA. During his postdoc in the group of David Baker at UW, he pioneered the development of general computational methods for the design of self-assembling proteins with atomic-level accuracy. He joined UW’s Department of Biochemistry and Institute for Protein Design as a Translational Investigator in 2014 before transitioning to Assistant Professor in July 2017. His group uses and extends computational methods to design functional protein nanomaterials for applications in structure-based vaccine design and targeted delivery of biologics.
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Postdoctoral Fellows

herpoldt@uw.edu

Karla-Luise Herpoldt, PhD

Karla is a Washington Research Foundation Innovation Fellow working with the King and Stayton Labs at UW. Her research focuses on the adaptation of protein nanomaterials for applications in drug delivery and vaccine development. Specific projects involve designing ‘smart’ protein materials that exhibit a strong response to environmental pH, engineering them to package synthetic polymer-prodrug carriers, and developing next-generation nanomaterials which will allow vaccines targeting the gastrointestinal mucosa to be delivered subcutaneously. She also enjoys complaining about the Seattle weather.

thuber@uw.edu

Thaddaus Huber, PhD

Thaddaus is a Washington Research Foundation Innovation Fellow. He is focused on developing atomically precise materials to support lipid bilayers. Additionally, he is interested in pursuing protein design and synthetic biology in non-conventional organisms.

akhmelin@uw.edu

Alena Khmelinskaia, PhD

Alena is generally intrigued by lipid membranes and their complex interactions with proteins. Her research is focused in how can a protein cage be singularly enveloped by a lipid membrane. In order to achieve a more malleable interface with the lipid membranes, she is introducing protein structural flexibility as a cage design principle. Her free time is passed in the company of her cat and piano.

Jake Kraft, PhD

How do pathogens avoid the immune systems of their mammalian hosts? Could these avoidance mechanisms be designed into novel protein nanomaterials to manipulate the immune response? These questions are at the heart of Jake’s research. His work aims to understand the immune-avoidance mechanisms that have evolved in pathogens in nature, and then translate these mechanisms into the design of novel protein nanoparticle vaccines and therapeutics to control/reduce their immunogenicity. He’s also employing covalent modification strategies the biopharmaceutical industry has used to shield immunogenic epitopes and reduce the clearance of protein therapeutics. Overall, his goal is to establish principles for evading and focusing the immune response to protein nanomaterials.

Aaron Sciore, PhD

Aaron is developing next-generation self-assembling vaccines for respiratory diseases. He is also developing general methods for optimizing the secretion of self-assembling vaccines from mammalian cells.


Graduate Students

Chloe Adams

Chloe is a Biochemistry PhD student. She is broadly interested in vaccine design and drug development. Specifically, she aims to design proteins to activate aspects of the innate immune system.

Jung-Ho Chun

Jung-Ho is a BPSD graduate student in the King Lab and the Baker Lab. He is interested in B cell engineering and vaccine design. He is currently working on designing de novo proteins that can direct B cells to specific cell stages, activation, and are useful in vaccine development.

Annie Dosey

Annie is a BPSD graduate student with a background in structural biology. She is pursuing the creation of a nanoparticle-based universal influenza vaccine, as well as a deeper understanding of how to control the immune response induced by the vaccine. Annie is also using both structural and computational methods as a means to probe nanoparticle formation and improve upon the accuracy and breadth of their design.

Quinton Dowling

Quinton works to increase the functionality and translational applications of protein nanoparticles by developing particles that interact with new materials, biological functions, and signals. His work can be broken down into computational design and assay development. Design goals include i) extending the types of protein nanomaterials accessible to design by generating quasi-equivalent and pseudosymmetric interfaces and ii) producing molecules of value both as basic research tools and as potential translational candidates by altering enzyme function. To facilitate evaluation of these designed proteins, Quinton develops biophysical assays and library-based or high-throughput methods for measuring function.

Dan Ellis

What is a pentamer? Are they our greatest adversaries or uncherished friends? Do they require soft nurturing or harsh discipline? Dan’s research is spent trying to answer such important questions. Beyond this, Dan works on computational development of novel methods of protein-protein interface design to build highly-programmable protein nanomaterials. In addition, he leads the development of nanocage-based vaccines for influenza within the King Lab. Dan is also adapting yeast-display methods to work towards building high-throughput screens to streamline properties of existing nanocages. In his free time, he enjoys playing lacrosse and taming stray cats.

Dan Humphrys

The activity of biological membranes is determined largely by transmembrane proteins. Dan is working to develop general methods for functionalizing the group’s EPN platform by recruiting specific membrane proteins to EPN membranes. This fundamental capability will enable a wide variety of applications in delivery, diagnostics, and structural biology.

Audrey Olshefsky

Audrey is a Bioengineering PhD student working in the King Lab and the Pun Lab. She is interested in targeted delivery of designed proteins with applications in immunotherapy. To that end, Audrey is developing new methods for in vivo and in vitro library selection based on synthetic protein nanocages.

John Wang

John is a student in the Molecular and Cellular Biology PhD program. With a background in chemical biology and biophysical chemistry, he looks to augment protein design methods by informing them with existing empirical results. These methods can then be applied to the development of novel protein technologies, from research platforms to potential therapeutics. John has a particular interest in the precision with which proteins are able to assemble, and seeks to understand the fundamental driving forces of protein folding and assembly through computation-guided experiments.

Dane Zambrano

Dane is a Biochemistry PhD student. She is interested in developing a platform of nanocages that can display patches of lipid membranes using her previous knowledge in nanodiscs. Her long term goal would be to be able to display de novo and native membrane proteins on the surface of nanocages for antigen display.

Research Scientists

Brooke Fiala

Brooke develops next-generation protein nanoparticle vaccines for respiratory diseases. She leads the group’s efforts to produce vaccine candidates comprising RSV F, the fusion protein from Respiratory Syncytial Virus, which causes severe respiratory disease in the very young, the elderly, and immunocompromised individuals. She is also working on nanoparticle vaccines for other seasonal viruses that cause respiratory pathologies. In addition to her research, Brooke is the lab manager of the King Lab and her spirit animal is the Success Cat.

Neil Gerstenmaier

Neil works with Quinton to produce and characterize novel classes of self-assembling protein nanomaterials.

Cassie Ogohara

Cassie works on developing and characterizing various protein nanomaterials for applications in targeted drug delivery and treatment of disease. Her work includes developing the group's Enveloped Protein Nanocage (EPN) platform for delivery of biologics. She helps manage the group's tissue culture space with Kate DaPron, facilitating and maintaining cell lines as well as training new lab members in a BL2 environment. Cassie is committed to creating cohesive community, celebrating cutting-edge creative constructs, cultivating a cosmic camp counselor culture and collectively curing cancer by characterizing cutting-edge computationally-designed cages. Cassie’s spirit animal is the Success Squirrel.

Isaac Sappington

Isaac’s projects focus on the dynamics of nucleic acid packaging using engineered, self-assembling proteins capable of in vitro assembly and nucleic acid binding. These nanomaterial variants have been optimized for these functions through a combination of several engineering methods, including computational modeling, directed evolution, and rational design. The research explores which features in proteins are necessary to exhibit high yields of nucleic acid encapsulation and protection from nucleic acid-degrading environments. These synthetic, virion-like nanomaterials may eventually be applied therapeutically to increase biological half-life and cellular uptake.

Adam Wargacki

Protein cages are a promising platform for display and delivery of therapeutic molecules. However, their biophysical properties must be mapped and optimized to enable predictability, uniformity, and stability throughout manufacturing and deployment. To this end, Adam is investigating the stoichiometry of cage assembly products, the stability of protein cages and their components, the dynamics and kinetics of assembly/disassembly, and the affinities of computationally designed nanomaterial interfaces. We have found that remarkably efficient assembly leads to protein nanomaterials exhibiting enhanced stability compared to their unassembled building blocks. These features present a promising profile for use as vaccine and drug delivery platforms, and may also hint that the emergence of such properties in designed nanomaterials is an intrinsic feature of higher-order protein complexes. Adam is an exceedingly private person, often hiding in his 1000-tree apple and pear orchard, a complex biological assembly he tends together with his wife and son.

Undergraduate Researchers

Rose Fields

Rose works with Karla to develop next-generation protein nanomaterials for induction of mucosal immunity via subcutaneous injection.

Gargi Kher

Gargi works with Karla on making mutations to a component of a protein cage in order for it to express solubly.

 

Margaux Randolph

Margaux works with Thaddaus to develop atomically precise protein lipid hybrid materials.

  

Chelsea Shu

Chelsea is currently a sophomore majoring in biochemistry at UW. She is working towards creating self-assembling protein platforms to improve mRNA vaccines.

 

Lab Alumni

Postdocs 

2016–2019 Carl Walkey (VP, Corporate Development at Neoleukin Therapeutics)

 

Research Scientists

2014–2015 Sueyeon Yi (MSTP program, University of Wisconsin, Madison, WI)
2014–2016 Dan Ellis (Graduate Program in MCB, University of Washington, Seattle, WA)
2015–2017 Julia Burrows (MSTP program, UCLA, Los Angeles, CA)
2015–2018 Kate DaPron (Graduate Program, CU Boulder, CO)

Undergraduate Researchers

2014–2015 Rachel Park (Ph.D. program in Biochemistry, Stanford University, Stanford, CA)
2014–2018 Hyung Chan (Brian) Kim (Medical School, Washington State University, Spokane WA)
2016–2018 Phong Ong
2018–2018 Ariel Stiber (Caltech SURF program)
2018–2019 Annamika Kannan (transferred to University of Michigan)
2019–2019 Conlan Olson (summer student)